Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: Flow chart of the study population. ARF: acute respiratory failure; BFPP: BioFire® FilmArray® Pneumonia Panel; mPCR: multiplex polymerase chain reaction; ICU: intensive care unit; VAP: ventilation-acquired pneumonia

Article Snippet: ARF: acute respiratory failure; BFPP: BioFire® FilmArray® Pneumonia Panel; mPCR: multiplex polymerase chain reaction; ICU: intensive care unit; VAP: ventilation-acquired pneumonia The population was predominantly male, with a median age of 65 yo, a median SAPSII of 53 and a SOFA score of > 8 in more than half the patients (Table ).

Techniques: Multiplex Assay, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: Incidence of bacteria detected by each of the 2 techniques BFPP mPCR: BioFire® FilmArray® Pneumonia Panel; CC: conventional culture; CNS: coagulase-negative Staphyloccocus ; PCR: polymerase chain reaction a Streptococcus except S.pneumoniae, S.agalactiae and S.pyogenes

Article Snippet: ARF: acute respiratory failure; BFPP: BioFire® FilmArray® Pneumonia Panel; mPCR: multiplex polymerase chain reaction; ICU: intensive care unit; VAP: ventilation-acquired pneumonia The population was predominantly male, with a median age of 65 yo, a median SAPSII of 53 and a SOFA score of > 8 in more than half the patients (Table ).

Techniques: Bacteria, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: PCR and conventional culture results for BFPP panel bacteria. PCR: polymerase chain reaction; CC: conventional culture; TP: true positive; FP: false positive; FN: false negative; TN: true positive

Article Snippet: ARF: acute respiratory failure; BFPP: BioFire® FilmArray® Pneumonia Panel; mPCR: multiplex polymerase chain reaction; ICU: intensive care unit; VAP: ventilation-acquired pneumonia The population was predominantly male, with a median age of 65 yo, a median SAPSII of 53 and a SOFA score of > 8 in more than half the patients (Table ).

Techniques: Bacteria, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: Flow chart of the study population. ARF: acute respiratory failure; BFPP: BioFire® FilmArray® Pneumonia Panel; mPCR: multiplex polymerase chain reaction; ICU: intensive care unit; VAP: ventilation-acquired pneumonia

Article Snippet: In this retrospective, single-center study, we analyzed data from critically ill immunocompromised patients admitted for acute respiratory failure requiring invasive ventilation, in whom a respiratory specimen was taken and processed simultaneously by BioFire FilmArray Pneumonia Panel multiplex PCR (BFPPm PCR) and conventional culture (CC).

Techniques: Multiplex Assay, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: Incidence of bacteria detected by each of the 2 techniques BFPP mPCR: BioFire® FilmArray® Pneumonia Panel; CC: conventional culture; CNS: coagulase-negative Staphyloccocus ; PCR: polymerase chain reaction a Streptococcus except S.pneumoniae, S.agalactiae and S.pyogenes

Article Snippet: In this retrospective, single-center study, we analyzed data from critically ill immunocompromised patients admitted for acute respiratory failure requiring invasive ventilation, in whom a respiratory specimen was taken and processed simultaneously by BioFire FilmArray Pneumonia Panel multiplex PCR (BFPPm PCR) and conventional culture (CC).

Techniques: Bacteria, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: Flow chart of the study population. ARF: acute respiratory failure; BFPP: BioFire® FilmArray® Pneumonia Panel; mPCR: multiplex polymerase chain reaction; ICU: intensive care unit; VAP: ventilation-acquired pneumonia

Article Snippet: Though BioFire ® FilmArray ® Pneumonia Panel (BFPP) mPCR has been shown to have a good diagnostic performance in immunocompetent critically ill patients, little data exist in immunocompromised patients [ ].

Techniques: Multiplex Assay, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: Incidence of bacteria detected by each of the 2 techniques BFPP mPCR: BioFire® FilmArray® Pneumonia Panel; CC: conventional culture; CNS: coagulase-negative Staphyloccocus ; PCR: polymerase chain reaction a Streptococcus except S.pneumoniae, S.agalactiae and S.pyogenes

Article Snippet: Though BioFire ® FilmArray ® Pneumonia Panel (BFPP) mPCR has been shown to have a good diagnostic performance in immunocompetent critically ill patients, little data exist in immunocompromised patients [ ].

Techniques: Bacteria, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: PCR and conventional culture results for BFPP panel bacteria. PCR: polymerase chain reaction; CC: conventional culture; TP: true positive; FP: false positive; FN: false negative; TN: true positive

Article Snippet: Though BioFire ® FilmArray ® Pneumonia Panel (BFPP) mPCR has been shown to have a good diagnostic performance in immunocompetent critically ill patients, little data exist in immunocompromised patients [ ].

Techniques: Bacteria, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Assessing FilmArray Pneumonia+ panel dynamics during antibiotic treatment to predict clinical success in ICU patients with ventilated hospital-acquired pneumonia and ventilator-associated pneumonia: a multicenter prospective study

doi: 10.1186/s13054-025-05503-7

Figure Lengend Snippet: Waterfall plot of the greatest change in bacterial load from diagnosis (Day 0) to Day 3 in ICU patients with vHAP/VAP, and their corresponding clinical outcome. ( A ) Changes in DNA copies/ml quantified by FA-PP in 71 patients. ( B ) Changes in CFU/ml quantified by conventional culture in 58 patients. Data are derived from Table . A Each patient is represented by a flow line according to the change in bacterial load from Day 0 to Day 3, classified into five categories: +2 log or more, + 1 log, no change, − 1 log, and − 2 log or less. The right column indicates the clinical outcome (success or failure). Bacterial load was assessed for the most abundant bacterium at Day 0. In cases of equal abundance, the most pathogenic was selected FA-PP FilmArray ® Pneumonia + panel; CFU colony-forming unit

Article Snippet: Among these, the FilmArray ® Pneumonia + panel (FA-PP, BioFire, bioMérieux) stands out for its rapid turnaround time of 75 min.

Techniques: Biomarker Discovery, Derivative Assay

Journal: Critical Care

Article Title: Assessing FilmArray Pneumonia+ panel dynamics during antibiotic treatment to predict clinical success in ICU patients with ventilated hospital-acquired pneumonia and ventilator-associated pneumonia: a multicenter prospective study

doi: 10.1186/s13054-025-05503-7

Figure Lengend Snippet: Proportion of bacteria detected at culture threshold categorized by FA-PP semi-quantitative results (DNA Copies/ml) for initial (Day 0) and follow-up samples. Culture diagnostic thresholds were defined as follows: BAL ≥ 104 CFU/ml, mini-BAL ≥ 103 CFU/ml, ETA ≥ 105 CFU/ml. For each FA-PP semi-quantitative category (DNA copies/ml), the proportion of bacteria detected at or above the culture threshold is shown by sample type and timepoint: Day 0 for BAL, mini-BAL, and ETA ( A – D ), and follow-up ETA ( E ) FA-PP FilmArray® Pneumonia+ panel, BAL bronchoalveolar lavage, CFU colony-forming unit, D0 Day 0

Article Snippet: Among these, the FilmArray ® Pneumonia + panel (FA-PP, BioFire, bioMérieux) stands out for its rapid turnaround time of 75 min.

Techniques: Bacteria, Diagnostic Assay